In Vitro Evaluation of Antioxidant Activity and Radical Scavenging Activity of Sesame Bioactive Peptides

Present study highlights on isolation of bioactive peptide from the sesame protein hydrolysate with an objective of better utilization sesame meal. Sesame protein isolate was enzymatically modified to prepare sesame protein hydrolysate using enzyme ‘Protease A Amano 2G’ in an enzyme membrane reactor and the hydrolysate was subsequently fractionated by ultrafiltration using membranes of decreasing molecular weight cut off. Peptide fractions were evaluated for their inhibitory effect on lipid peroxidation and also assayed for their antiradical activity in several in vitro models. Peptide fraction of molecular mass <1kDa was found most potent compared to other fractions. Mass spectra analysis (MS-TOF) detected the presence of six peptides. Overall findings suggest the possibility of getting bioactive peptides from sesame meal, which can be utilized for enhancing product self life as an oxidation inhibitor. Keywords— Bioactive peptide, enzyme membrane reactor, lipid peroxidation, radical scavenging, ultrafiltration.